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Hyperinsulinemic and GlcN states induce a loss in PM PIP2 detection and cortical F-actin. Representative immunofluorescent (IF) images of PIP2 (panels 1–4) and WGA (panels 5–8) detected in PM sheets treated as described in the preceding figures are shown. Phalloidin stained F-actin and propidium iodide labeled nuclei in these cells (panels 9–12) are shown. Images are representative from eight to ten independent experiments.
