Figure 6.

Reduced Treg suppressive potency after Lm infection. A. Purification of Foxp3⁺ Tregs as GFP⁺ CD4⁺ cells in Foxp3^GFP reporter mice after Lm infection. Percent GFP or Foxp3 expression among CD4⁺ splenocytes at each time point after infection before sorting (Pre-sort) and among all cells after sorting (Post-sort) for GFP⁺ CD4⁺ cells. B. CFSE dilution among CD8⁺ CD45.1⁺ responder cells after co-culture with GFP⁺ Tregs at the indicated ratio and stimulation with anti-CD3 antibody (line histogram), no stimulation controls (dark filled histogram), or stimulation without Tregs (light filled histogram). C. Percent CD8⁺ CD45.1⁺ responder cells in each CFSE generation after co-culture with GFP⁺ Tregs from mice at the indicated time point after infection at a 1:8 ratio. These data are representative of three experiments containing Tregs from 6–8 mice per infection time point. Bar, one standard error.