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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Aug 1;187(5):2569–2577. doi: 10.4049/jimmunol.1100374

Figure 7.

Figure 7

Antigen-pulsed dendritic cells do not override the impacts of Treg-suppression. A. Relative expression of MHC-I (H-2Kb) or CD80 on CD11c+ cells isolated directly ex vivo from naïve Treg sufficient mice (gray filled histogram and symbols), or mice day 5 after Lm infection (red line histogram and symbols) or ablation of Foxp3+ Tregs (blue line histogram and symbols) compared with JAWS II cells maintained in vitro (black line histogram and symbols). B. Percent and number of OVA-specific CD90.1+ CD8+ T cells among splenocytes day 5 after stimulation with peptide pulsed or unpulsed JAWS II dendritic cells in the presence (Foxp3-WT) or absence of Tregs (Foxp3-DTR). Expression of CD25 and IFN-γ production by CD90.1+ (line histogram) or bulk CD8+ T cells (filled histogram) day 5 after peptide stimulation in Treg-sufficient (Foxp3-WT) or mice ablated of Tregs one day before and on the day of peptide inoculation (Foxp3-DTR). These data are representative of three experiments containing 8–12 mice per group. Bar, one standard error.