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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Jul 29;187(5):2162–2171. doi: 10.4049/jimmunol.1002906

Figure 2.

Figure 2

Allelic Polymorphism In KIR3DL1 Uncovers Amino Acid Variations In D1 Domain That Differentially Influences DX9 And Z27 Recognition. (A) HEK293T cells were transfected with increasing concentration of plasmid expressing FLAG tagged KIR3DL1*01502 (squares), KIR3DL1*054 (upright triangle) or KIR3DL1*01502 G138W (inverted triangle) and stained with anti-FLAG, DX9 and Z27 antibodies. The mean fluorescent intensity of the positive population is shown. (B) As above with plasmids containing FLAG-tagged KIR3DL1*01502 (squares), *01502 P163S (upright triangles) and *01502 L166R (inverted triangles). Data are expressed as DX9 or Z27 MFI relative to the anti-FLAG MFI of the total cell population to normalize for variation in cell surface expression due to transfection efficiency [e.g. for *01502 1 μg DNA: FLAG MFI 26, DX9 MFI 129, Ratio 5.0]. (C) HEK293T cells were transfected with increasing concentration of plasmid expressing FLAG tagged KIR3DS1 (closed squares), KIR3DS1 W138G (triangle), KIR3DS1 S163P (open squares), KIR3DS1 R166L (diamonds) or KIR3DS1 L199P (circles) and analyzed as in (A). (D) A comparison of the staining profile of transfected HEK293T cells stained with anti-FLAG and DX9 (top panel) and Z27 (bottom panel) antibodies. Results are representative of findings made in at least two independent experiments.