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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Jul 29;187(5):2162–2171. doi: 10.4049/jimmunol.1002906

Figure 4.

Figure 4

Reversion Of W138 Of KIR3DS1 To The G138 Of KIR3DL1 Allows Binding Of HLA. (A) HEK293T cells were transfected with 2 μg of plasmid containing FLAG tagged KIR3DS1 W138G and stained with PE-conjugated HLA tetramers. Cells were pre-incubated with Z27 or mouse IgG1 (10 μg/ml) for ten minutes prior to staining. (B) Jurkat cell lines expressing KIR3DS1*013 or KIR3DS1*014 extracellular domain fused to the CD3 zeta chain were stimulated with Z27 antibody or 721.221 cell line transfectants at a 1:1 stimulator to responder ratio. Luciferase activity was measured after 18 hours as a measure of NFAT activity and normalized to unstimulated control. Data shown is representative of n=2 (KIR3DS1*013) or n=4 (KIR3DS1*014) independent experiments.