. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: J Immunol. 2011 Jul 29;187(5):2162–2171. doi: 10.4049/jimmunol.1002906

Table I.

Mutagenesis Primers

Primer sequences used for the mutagenesis of KIR3DL1 and KIR3DS1 constructs

	Change		Primer Sequence (Forward and Reverse)
	Amino Acid	Nucleotide	Primer Sequence (Forward and Reverse)
KIR3DL1 *01502	G138W	G475T	CTGCACAAAGAGTGGATCTCTAAGGACCCC
	G138W	G475T	GGGGTCCTTAGAGATCCACTCTTTGTGCAG
	P163S	C550T	CAATTTCTCCATCGGTTCCATGATGCTTGCCC
	P163S	C550T	GGGCAAGCATCATGGAACCGATGGAGAAATTG
	L166R	T560G	CGGTCCCATGATGCGTGCCCTTGCAGGG
	L166R	T560G	CCCTGCAAGGGCACGCATCATGGGACCG
KIR3DS1	W138G	T475G	CTGCACAAAGAGGGGATCTCTAAGGACCCC
	W138G	T475G	GGGGTCCTTAGAGATCCCCTCTTTGTGCAG
	S163P	T550C	CAATTTCTCCATCGGTCCCATGATGCGTGCCC
	S163P	T550C	GGGCACGCATCATGGGACCGATGGAGAAATTG
	R166L	G560T	CGGTTCCATGATGCTTGCCCTTGCAGGG
	R166L	G560T	CCCTGCAAGGGCAAGCATCATGGAACCG
	L199P	T659C	CATCGTGGTCACAGGTCCATATGAGAAACCTTCTC
	L199P	T659C	GAGAAGGTTTCTCATATGGACCTGTGACCACGATG
KIR3DS1 _S163P	R166L	G560T	GGTCCCATGATGCTTGCCCTTGCAGGG
KIR3DS1 _S163P	R166L	G560T	CCCTGCAAGGGCAAGCATCATGGGACC