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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Jul 22;187(5):2484–2491. doi: 10.4049/jimmunol.1100468

Figure 1.

Figure 1

Influence of Eδ on Tcra/Tcrd locus histone modifications in adult DN thymocytes. A. Map of the Tcra/Tcrd locus depicting the relative positioning of gene segments analyzed in this study. Enhancers Eδ and Eα (circles), and the D2 promoter (bent arrow) are also depicted. Not all Tcra/Tcrd gene segments are shown. H3ac (B), H4ac (C), H3K4me2 (D) and H3K4me3 (E) were measured by ChIP using chromatin prepared from Rag2−/− and Eδ−/−Rag2−/− thymocytes. TRDD1+01 and TRDJ1+01 are sites situated 1kb downstream of D1 and J1, respectively. The data represent the mean ± SEM of three independent chromatin preparations for each genotype. Values of bound/input were expressed relative to those for B2m (normalized to one) in each sample. Note that PCR for TRAV11 detects both TRAV11 and TRAV11D; PCR for TRAV14 detects six members of the TRAV14 family (TRAV14D-1, D-2, D-3,-1,-2,-3). The significance of differences between Eδ−/− and wild-type were evaluated by two-tailed Student’s t-test: *, P<.01.