Figure 2.

Influence of E_δ on Tcra/Tcrd locus germline transcription in adult DN thymocytes. A. Germline transcription was measured by RT-PCR using serial three-fold dilutions of cDNA (wedges) prepared from four independent cDNA preparations from Rag2^−/− and E_δ^−/−Rag2^−/− thymocytes. Two preparations for each genotype are analyzed in the top set of panels; two different preparations for each genotype are analyzed in the bottom set of panels. (−) no reverse transcriptase. The TRAV14 and TRAV15 primers detect all members of the TRAV14 and TRAV15 families. B. Real-time PCR of germline transcription using cDNA preparations from Rag2^−/− and E_δ^−/−Rag2^−/− thymocytes. The data represent the mean ± SEM of five independent cDNA preparations for each genotype, all normalized to values for β-actin (Actb). The values for E_δ^−/−Rag2^−/− for each site were then expressed relative to those for Rag2^−/−, which were normalized to one. The significance of differences between E_δ^−/− and wild-type were evaluated by two-tailed Student’s t-test: *, P<.05.