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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Jul 22;187(5):2484–2491. doi: 10.4049/jimmunol.1100468

Figure 5.

Figure 5

H3K9 dimethylation of Vδ gene segments in adult and fetal thymocytes. A. H3K9me2 of TRDV2-2, TRDV4 and TRDV5 was measured by ChIP using chromatin prepared from adult Rag2−/− thymocytes. Each V gene segment was analyzed at three sites (a, b, c, as diagrammed. Values of bound/input were expressed relative to MageA2 (normalized to one). The data represent the mean ± SEM of three independent chromatin preparations. B. H3K9me2 was compared in chromatin prepared from F17.5 and adult Rag2−/− thymocytes. The data represent the mean ± SEM of three to four independent chromatin preparations. TRDV4 and TRDV5 were analyzed at sites “b”. 6.9UDB is a positive control site within the Tcrb locus (39); B2m and Actb served as negative control sites.