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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Jul 25;187(5):2148–2154. doi: 10.4049/jimmunol.1101180

Figure 2.

Figure 2

Ag-specific CD8 T cell responses to attenuated LM-OVA are reduced in CLP-treated WT B6 mice. WT B6 mice underwent sham or CLP surgery, and were then infected with 107 CFU attenuated LM-OVA on d 2 post-surgery. A. Total CD8 T cell numbers in the spleen were determined on d 2 post-surgery (i.e. day of infection). B–D. On d 7 after infection, the number of Ag-experienced (based on CD11a upregulation) and OVA257-specific CD8 T cells in the spleen were determined. The total number of Ag-experienced CD11ahi CD8 T cells in spleen was determined directly ex vivo by flow cytometry, while the number of OVA257-specific CD8 T cells in the spleen were determined by intracellular cytokine staining for IFN-γ afterin vitro restimulation with OVA257. Panel C shows representative flow plots measuring the frequency of IFN-γ-producing CD8 T cells with and without OVA257 restimulation in vitro. *p < 0.05 (Student’s t-test).