NO generation of a) (e-PEI/Alg)50 A) without annealing B) cross-link only C) after two-step annealing in 100 μM EDTA, 50 μM GSH, 50 μM GSNO at R.T., films are placed in and out of the NOA cell as indicated by ↓ and ↑ arrows. b) NO generation of LbL films with different bilayers on glass slides in 100 μM EDTA, 50 μM GSH, 50 μM GSNO at R.T. Insert: Se content of LbL films vs layer numbers. c) NO generation of (e-PEI/Alg)100 in 100 μM EDTA, 50 μM GSH, 50 μM GSNO at R.T. Film was repeatedly inserting and taken out of the solution as indicated by the ↑ and ↓arrows. d) NO generation from (e-PEI/Alg)50 on polyurethane catheters (insert) in 100 μM EDTA, 20 μM CySH, 10 μM CysNO at 37.5 °C after 24 h soaking in A) PBS B) sheep plasma C) sheep blood.