Table 3.
PHA biosynthesis by R. eutropha PHB-4 expressing phaC1Ps or leucine analog resistant mutants with the supplementation of 10 g/L leucine
| Dry cell weight (g/L) | PHA content (wt%) | PHA composition (mol%) a | |||
|---|---|---|---|---|---|
| Strain | 3HB | 3HV | 3H4MV | ||
| Parent b | 7.2 | 29 | 98.8 | 0.3 | 0.9 |
| 1F2 c | 7.1 | 34 | 95.8 | 1.2 | 3.0 |
| 6C1 c | 7.2 | 36 | 96.5 | 1.0 | 2.5 |
| 12D1 c | 7.3 | 35 | 95.8 | 1.4 | 2.8 |
| 13H3 c | 7.2 | 35 | 95.9 | 1.3 | 2.8 |
Cells were cultured in MS plus fructose (20 g/L) medium supplemented with L-leucine (10 g/L). The results are the averages of three independent cultivations (the standard deviations were less than 5% of the mean).
a PHA composition was determined by GC.
b R. eutropha PHB-4 expressing phaC1Ps.
c leucine analog resistant mutants.