Table 3.
Summary of SSCF of wheat straw with 7% WIS after 96 h showing concentrations and yields (mean values of duplicate experiments). The same conditions (temperature, pH, yeast- and enzyme loading) were used in all experiments.
| XR and Gxf1 expression (Strain) |
Xylose (g L-1) |
Xylitol (g L-1) |
Glycerol (g L-1) |
Ethanol (g L-1) |
Xylose consumptiona (%) |
Xylitol yieldb (%) |
Ethanol yieldc (g g-1) |
|---|---|---|---|---|---|---|---|
| Native XR (TMB3424) |
12.7 ± 0.9 | 2.5 ± 0.3 | 4.4 ± 0.1 | 22.2 ± 0.1 | 38 | 32 | 0.33 |
| Mutated XR (TMB3422) |
5.0 ± 0.6 | 2.1 ± 0.1 | 3.9 ± 0.3 | 26.2 ± 0.4 | 76 | 13 | 0.39 |
| Native XR+Gxf1 (TMB3426) |
11.8 ± 2.0 | 2.7 ± 0.8 | 4.1 ± 0.2 | 20.1 ± 0.1 | 42 | 31 | 0.30 |
| Mutated XR+Gxf1 (TMB3425) |
3.2 ± 0.5 | 2.4 ± 0.1 | 4.2 ± 0.1 | 25.9 ± 0.9 | 84 | 13 | 0.39 |
a. Based on to total amount of xylose (present both in the fibers and the liquid fraction).
b. Based on consumed xylose.
c. Based on total amount of available sugars (present both in the fibers and the liquid fraction).