Table 3.
Substrate | Kinetic constants | ||
---|---|---|---|
Vmaxa (units/mg) |
Km (mM) |
Vmax/Km | |
(±)-Alliin | 90.0 | 2.3 | 39.1 |
(+)-Alliin | 18.5 | 5.7 | 3.2 |
S-Allyl-L-cysteine | 66.1 | 10.6 | 6.2 |
S-Methyl-L-cysteine | 15.3 | -b | - |
(±)-S-Methyl-L-cysteine sulfoxide | 53.6 | - | - |
S-Ethyl-L-cysteine | 29.7 | - | - |
L-Cysteine | 1.6 | - | - |
a The Vmax value was obtained by the rate of pyruvic acid production in the standard reaction mixture containing each substrate at 40 mM.
b Not tested.