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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Prog Mol Biol Transl Sci. 2011;100:287–330. doi: 10.1016/B978-0-12-384878-9.00007-8

Figure 1. Chemical versus insertional mutagenesis screens in zebrafish.

Figure 1

In chemical screens (A), the mutagen ethynitrosourea (ENU) is used to induce mutations in the adult male germline. The mutagenized males (F0 generation) are then out-crossed to generate a heterozygous F1 population. F1 progeny are crossed again to wild-type fish, giving rise to F2 families that carry a specific mutation. F2 siblings are bred to each other in order to generate an F3 generation out of which 25% will be homozygous for the mutation [284, 285]. In an insertional screen (B), a retrovirus is injected into fertilized eggs to generate transgenic adults (F0) that will be subsequently bred to generate an F1 generation with multiple viral inserts. PCR and Southern blot analysis is then performed in order to identify F1 fish that carry multiple inserts. As in a chemical screen, F1 fish are bred to generate F2 families; those will be subsequently bred to siblings to give rise to F3 progeny that will be screened for physiological and/or behavioral phenotypes [40, 285].