Table 1.
Peptides Comprising Clp Protease Core Subunits Selected for Absolute Quantificationa
| Accession | Peptide | Sequence | m/zb | Qc |
| ATCG00670.1 | P1-1 | IAFPHAR | 271.1573 (+3) | N |
| P1-2 | SPGEGDTSWVDIYNR | 848.3841 (+2) | Y | |
| AT1G49970.1 | R1-1 | YLQAQAAIDYGIADK | 820.4199 (+2) | N |
| R1-2 | TAPPDLPSLLLDAR | 739.9143 (+2) | Y | |
| AT1G12410.1 | R2-1 | IALQSPAGAAR | 527.8038 (+2) | Y |
| R2-2 | FNAEEAIEYGLIDK | 806.3987 (+2) | Y | |
| AT1G09130.1 | R3-1 | EPIYIYINSTGTTR | 814.4199 (+2) | N |
| R3-2 | DILVELLSK | 515.3132 (+2) | Y | |
| AT4G17040.1 | R4-1 | GSAHEQPPPDLASYLFK | 619.6441 (+3) | Y |
| R4-2 | YFSPTEAVEYGIIDK | 866.4274 (+2) | Y | |
| AT1G66670.1 | P3-1 | DNTNLPSER | 523.2491 (+2) | N |
| P3-2 | LPSFEELDTTNMLLR | 889.9533 (+2) | Y | |
| P3-2 (ox) | LPSFEELDTTNM(ox)LLR | 897.9508 (+2) | Y | |
| AT5G45390.1 | P4-1 | SFEQVLK | 425.7371 (+2) | Y |
| P4-2 | ADVSTIALGIAASTASIILGAGTK | 1101.5790 (+2) | N | |
| AT1G02560.1 | P5-1 | ANLNGYLAYHTGQSLEK | 626.9830 (+3) | Y |
| P5-1 (d) | ANLDGYLAYHTGQSLEK | 627.3110 (+3) | Y | |
| P5-2 | FQSIISQLFQYR | 765.4092 (+2) | Y | |
| AT1G11750.1 | P6-1 | IIFIGQPINAQVAQR | 834.4832 (+2) | Y |
| P6-2 | VISQLVTLASIDDK | 751.4272 (+2) | Y |
a
For details on the other peptides that were concatenated within the Clp-QconCAT construct (includes peptides for Clp chaperones and adaptors), see Supplemental Table 2 online.
b
The m/z for the most abundant peptide species (unlabeled) with the charge state indicated in parentheses.
c
Can be consistently detected and quantified. Y, yes; N, no.