Ectopic Expression of Mt STF and Nb STF1 in N. sylvestris.
(A) Free IAA content in mature leaves of lam1 mutant, Wild type, STF:GUS transgenic control, and 35S:STF transgenic plant. Values are the mean and se of five replicates (***P < 0.001). FW, fresh weight.
(B) 35S:STF transgenic plant showing upward curling leaf phenotype.
(C) 35S:Nb-STF1 transgenic leaf showing upward curling phenotype.
(D) Wild-type leaf.
(E) Left, 4-week-old wild-type (WT) N. sylvestris; right, 17-month-old transgenic plant with highest STF overexpression showing shoot and root deformation.
(F) Close-up of the transgenic plant in (E) showing two large tumors. Scale bars = 5 cm.