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. 2011 Jun 14;23(6):2209–2224. doi: 10.1105/tpc.110.082651

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Figure 6. — ASPARTIC PROTEASE/UNDEAD Expression Analysis.

(A) to (D) Sections (3 μm) of UNDEAD promoter:GUS anthers stained with safranin. Light microscopy of anthers at stages 7 (A), 8 (B), 9 (C), and early 10 (D) shows tapetum- and microspore-specific expression. Weak GUS activity is present in stage 10 pollen grains. No GUS activity was detected in stage 11 and beyond.

(E) Comparative qRT-PCR analysis of UNDEAD transcript levels in wild-type mature (anther stages 10 to 12) versus young (anther stages ≤ 9) floral buds. The UNDEAD transcript level is lower in mature floral buds. Error bar represents sd. Tap, tapetum; Tds, tetrads; Mic, microspores; PG, pollen grain. Bars = 25 μm.