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. 2011 Jun 14;23(6):2209–2224. doi: 10.1105/tpc.110.082651

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Figure 8. — VGD1 Expression Analysis.

(A) to (D) Sections (3 μm) of VGD1 promoter:GUS anthers stained with safranin. Light microscopy of anthers at stages 10 (A) and 11 (B). Blue crystals are weakly present within the tapetum and developing pollen grains. Dark-field microscopy of anthers at stages 10 (C) and 11 (D). GUS activity is visualized as red crystals. GUS activity was not detected in stage 8 or earlier stages.

(E) Comparative qRT-PCR analysis of VGD1 transcript levels in wild-type mature (anther stages 10 to 12) versus young (anther stages ≤ 9) floral buds. The VGD1 transcript level is higher in mature floral buds. Error bar represents sd. Mic, microspores; Tap, tapetum; PG, pollen grain. Bars = 25 μm.