Table 1.
A Subset of Genes from the Inducible MYB80 Microarray Data Set Analyzed by ChIP
| Gene Locus ID | Name or Annotation | Predicted MYB Binding Motifs | Dex24 | ChIP |
| At4g12920 | Aspartic protease/UNDEAD | MYB1AT x8, MYBST1 x3, MYB1LEPR x2, MYBGAHV | 14.67 | + |
| At1g67290 | Glyoxal oxidase/GLOX1 | MYB1AT, MYBPZM | −2.91 | + |
| At2g47040 | PME/VANGUARD1 | MYB1AT x4, MYBCORE, MYBGAHV, MYBST1 | −29.19 | + |
| At1g01280 | Cytochrome p450 | MYBCORE x2, MYBGAHV x3, MYB1LEPR | 87.68 | − |
| At1g02790 | Polygalacturonase 4 | MYBCORE, MYBGAHV x2 | −21.22 | − |
| At1g02930 | Glutathione S-transferase, putative | MYB1AT x2, MYBGAHV, MYBST1, MYB1LEPR, MYBCOREATCYCB1 | −2.62 | − |
| At1g29270 | Expressed protein | MYB1AT | 13.14 | − |
| At1g54540 | late embryogenesis abundant (LEA) | MYB1AT, MYBCORE | 10.16 | − |
| At1g78140 | Methyltransferase | MYB1AT x3, MYBST1 | 2.08 | − |
| At2g02810 | UDP-galatose transporter 1 | MYB1AT x3, MYBCOREATCYCB1 | 2.53 | − |
| At2g03740 | LEA | MYB1AT, MYBGAHV x2, MYB1LEPR | 2.15 | − |
| At2g42570 | Expressed protein | MYB1AT x3 | 2.90 | − |
| At2g43520 | Trypsin inhibitor, putative | MYB1AT x2, MYBGAHV | 2.16 | − |
| At2g46660 | Cytochrome p450, putative | MYB1AT, MYBST1, MYBCOREATCYCB1 | 2.6 | − |
| At2g47050 | Invertase/PME inhibitor | MYB1AT x2, MYBCORE | −8.85 | − |
| At3g01700 | Arabinogalactan protein 11 | MYBST1 | −3.41 | − |
| At3g01290 | Band 7 family protein | MYB1AT, MYBST1 | −2.13 | − |
| At3g02480 | LEA, abscisic acid–responsive protein | MYB1AT, MYBCORE x2, MYBST1, MYBGAHV | −5.27 | − |
| At3g11980 | Male Sterility2 (MS2) | MYB1AT x3, MYBGAHV x2, MYB1LEPR | 24.85 | − |
| At3g23770 | Glycosyl hydrolase | MYB1AT, MYBCORE, MYB1LEPR, MYBPZM | 37.49 | − |
| At3g62180 | PME | MYBCORE, MYBGAHV, MYBCOREATCYCB1 x2, MYBPZM | −2.25 | − |
| At4g11760 | Pollen coat protein | MYB1AT x2, MYBCORE x2, MYBGAHV | −11.75 | − |
| At4g14080 | A6, putative callase/MEE48 | MYBPZM | <2 | − |
| At4g16160 | Inner membrane translocase | MYBCORE, MYBST1, MYBCOREATCYCB1 x2 | −4.27 | − |
| At4g22080 | Pectate lyase | 14.7 | − | |
| At4g30030 | Aspartic protease | MYB1AT, MYBCORE x2 | N/A | |
| At4g30040 | Aspartic protease | MYB1AT, MYBST1 | 9.85 | − |
| At5g09550 | Rab GDP dissociation inhibitor | MYB1AT, MYBCORE x2, MYBCOREATCYCB1 | −7.65 | − |
| At5g52160 | Protease inhibitor | MYBST1 | 5.97 | − |
| At5g22260 | Male Sterility 1 (MS1) | N/A | − | |
| At5g48880 | Acetyl-CoA C-acyltransferase 1 | MYBCORE, MYBST1 x2 | 3.23 | − |
| At5g56110 | MYB80/MYB103 | MYBST1, MYBGAHV x2, MYBPZM | <2 | − |
Predicted MYB binding motifs present in the promoters (up to −600 bp from the ATG) were obtained using cis-PLACE analysis. ChIP identified UNDEAD, GLOX1, and VGD1 as direct targets of MYB80. The aspartic protease At4g30030 was examined by ChIP as it occurs in tandem with At4g0040. MS1 is absent from the Affymetrix ATH1 array. A6 and MYB80 transcript difference was <2-fold. Dex24, differential gene expression (fold change) 24 h postfunctional MYB80 induction. MYB1AT, A/TAACCA (Abe et al., 2003); MYBCORE, CNGTTA/G (Urao et al., 1993); MYBST1, GGATA (Baranowskij et al., 1994); MYBGAHV, TAACAAA (Gubler et al., 1999); MYB1LEPR, GTTAGTT (Chakravarthy et al., 2003); MYBCOREATCYCB1, AACGG (Planchais et al., 2002); MYBPZM, CCA/TACC (Grotewold et al., 1994). N/A, not present on the ATH1 array. Primers are presented in Supplemental Table 5 online.