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. 2011 Sep 15;138(18):3879–3884. doi: 10.1242/dev.069336

Fig. 1.

Fig. 1.

Bam expression and function during hematopoiesis. (A) Immunostaining of wild-type (wt) Drosophila lymph glands with an anti-Bam antibody. (B) dome-lacZ serves as a marker of prohemocytes in wild-type lymph glands. (C) Reduction of dome-lacZ-positive prohemocytes in lymph glands from bamΔ86 larvae. (D) P1 antibody detects plasmatocytes in lymph glands from wild-type larvae. (E) Increased numbers of plasmatocytes in lymph glands dissected from bamΔ86 larvae. (F) Expression of BcF6-GFP marks crystal cells (present), whereas activity of MSNF9-mCherry marks lamellocytes (absent) in wild-type lymph glands. (G) Increased crystal cells and induced lamellocytes in lymph glands from bamΔ86 larvae. (H) Immunostaining with P1 antibody and domeGal4-driven UAS-2XEYFP expression in control lymph glands. (I) Forced expression of the UAS-bamGFP transgene by domeGal4 results in expansion of the medullary zone and reduction of plasmatocytes. CZ, cortical zone; MZ, medullary zone.