Fig. 5.
Lef1 function is required in leading edge cells of the primordium for proper pLL formation. (A-C′) Confocal projection obtained from a lef1nl2 mutant host that received wild-type donor cells (rhodamine dextran, red). (A) At 24 hpf, the primordium contains donor cells in the leading zone and caudal-most rosette. (B) The same embryo as in A, showing complete primordium migration and tc formation at 48 hpf. (B′) High magnification of region outlined in B; the primordium is entirely composed of donor cells. (C) Lateral line is truncated on the contralateral side of the same embryo. (C′) High magnification of the region outlined in C. Both wild-type donors and mutant hosts expressed Tg(–8.0cldnb:lynGFP) transgene. Scale bars: 20 μm.