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. 2011 Sep 15;138(18):3921–3930. doi: 10.1242/dev.062554

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Fig. 6. — Wnt signaling regulates proliferation in the primordium. (A-C′′) Confocal projections of primordia following BrdU incorporation between 32.5 and 34.0 hpf in wild-type, lef1^nl2 mutant and Tg(hsp70l:dkk1-GFP) embryos. All embryos express Tg(–8.0cldnb:lynGFP). BrdU incorporation in the primordia of the wild-type (A-A′′), the lef1^nl2 mutant (B-B′′) and the Tg(hsp70l:dkk1-GFP) transgenic embryos heat-shocked at 28 hpf (C-C′′). Scale bar: 20 μm. (D,E) BrdU incorporation index for wild-type sibling and lef1^nl2 mutants (D) and control and Tg(hsp70l:dkk1-GFP) embryos (E). (F,G) BrdU incorporation index for leading region in wild-type siblings and lef1^nl2 mutants (F) and controls and Tg(hsp70l:dkk1-GFP) embryos (G) (n=10-22 embryos; data presented as mean±s.e.m., ^**P<0.009, ^***P<0.002, Student's t-test). The leading region is defined as the cells posterior to the leading rosette in the primordium.