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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Dev Neurobiol. 2011 May;71(5):362–373. doi: 10.1002/dneu.20869

Figure 2.

Figure 2

Enteric neurons in the wild-type stomach are netrin-immunoreactive at E12-E16. Stomach from E12 and 16 fetal mice was immunostained to locate netrin-1 (red; A, D) and the neuronal marker, PGP9.5 (green; B, E) in the bowel wall. Nuclei are identified by staining DNA with bisbenzimide (blue). The lumen of the stomach is labeled (lumen) and the images are merged in C and F. (A-C; E12) Netrin-1 immunoreactivity (A, C) is found in the mucosal epithelium and outer mesenchyme (labeled in C). PGP9.5 immunoreactivity (B) overlaps the netrin-immunoreactive band in the outer mesenchyme. At high magnification, a subset of neurons (B; arrow and inset) are also netrin-1-immunoreactive (A, C; arrow, box and inset). (D-F; E16) The gastric mucosal epithelium and outer mesenchyme (labeled in F) are still netrin-1-immunoreactive (D, F). PGP.5 immunoreactivity corresponds in location to the band of netrin-1 immunoreactivity in the outer mesenchyme (E, F). At high magnification, many neurons (E; arrow and inset) are also netrin-1-immunoreactive (E, F; arrow, box and inset). Bars = 25 μm (B, E); 5 μm (B; inset); 15 μm (E; inset).