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. 2011 Jan 12;31(2):764–774. doi: 10.1523/JNEUROSCI.5052-10.2011

Figure 8.

Figure 8.

Nr4a2 siRNA attenuates the long-term memory enhancement observed in HDAC3flox/flox mice. A, At 48 h after infusions of Nr4a2 or RISC-free siRNA, HDAC3flox/flox and HDAC3+/+ mice received subthreshold training (3 min) in an environment with two identical objects and received a retention test 24 h later in which one object is moved to a new location. B, HDAC3flox/flox mice infused with RISC free (n = 10) exhibited significant memory for object location compared with HDAC3+/+ mice (††p = 0.001), which was blocked by Nr4a2 siRNA treatment (n = 9–10 per group; **p < 0.001). C, At 2 h after testing, quantitative RT-PCR shows that Nr4a2 siRNA treatment significantly reduced Nr4a2 expression in both HDAC3flox/flox and HDAC3+/+ mice (n = 3 per group; **p < 0.001 and *p < 0.05 vs respective RISC-free siRNA controls). HDAC3flox/flox mice also exhibited an increased induction of Nr4a2 mRNA after the long-term memory test (††p = 0.002 vs HDAC3+/+ RISC free).