Figure 3.

Repeated social defeat increased the percentage of CD11b⁺/ CD45^high/Ly6C^high macrophages in the CNS. Male C57BL/6 mice were injected subcutaneously with vehicle or propranolol (10 mg/kg) before each of the six cycles of SDR. Brains were collected 14 h after the sixth cycle of social defeat, CD11b⁺ cells were enriched by Percoll gradient separation, and CD11b, CD45, and Ly6C expression levels were determined through flow cytometry. A, Representative bivariate dot plots of CD11b/CD45 staining. B, Average number of CD11b⁺/CD45^high macrophages (n = 10). C, Representative bivariate dot plots of Ly6C/CD45 staining. D, Average number of CD11b⁺/CD45^high/Ly6C^high macrophages detected (n = 5). Bars represent the mean ± SEM. Means with different letters (a or b) are significantly different (p < 0.05) from each other. E, In a related study, mice were subjected to repeated social defeat and half the mice were perfused before collecting brains for flow cytometric analysis (n = 6). Bars represent the mean ± SEM. Means with different letters (a or b) are significantly different (p < 0.05) from each other. APC, Allophycocyanin; PerCP, peridinin chlorophyll protein.