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. 2011 Jun 3;30(14):2843–2852. doi: 10.1038/emboj.2011.181

Figure 1.

Figure 1

SAGA mutants show a range of phenotypes under different stress conditions. Growth phenotypes of SAGA mutants compared with an isogenic wild-type strain. Cells were grown in liquid YES medium to stationary phase, subjected to 10-fold serial dilutions and spotted onto test plates, as indicated. The growth phenotypes observed on rich medium (YES), supplemented with 3% raffinose, 15 mM caffeine, 1 M KCl, 10 mM hydroxyurea (HU), or incubated at 38°C, as well as on minimal medium (EMM) containing 0.02 μg/ml rapamycin, or 20 μg/ml canavanine (see Supplementary Table 2 for details) are shown here. All plates were incubated at 32°C for 3 days, except caffeine, KCl, and HU plates, which were incubated for 6 days. All strains were spotted onto one plate, but arranged in two separate panels for constructing the figure. The results from one representative experiment out of four independent replicates are shown.