Figure 3.

Tra1 is not required for the integrity of the SAGA complex. The Ada1–TAP protein was purified from wild-type (tra1⁺) and tra1Δ strains. Co-purifying proteins were separated on an SDS 5–20% gradient polyacrylamide gel and visualized by silver staining. An untagged control strain is shown (‘no tag’). The positions of molecular weight markers (MW) are shown on the right. The mass spectrometry analysis of these purifications is presented in Supplementary Table 3.