Figure 5.

Effects of a tra1Δ mutation on SAGA recruitment at distinct genes. Spt7 occupancy was assessed by ChIP analysis of chromatin extracts from strains in which Spt7 was tagged with 13 copies of a Myc epitope, either in a wild-type (dark grey) or in a tra1Δ (light grey) background. Cells were grown to mid-log phase in minimal medium, in the presence of nutrients. Occupancy levels were quantified by real-time PCR of ‘IP’ over ‘input’ DNA and normalized to background levels detected at a non-transcribed region (mat3). Spt7 binding was specifically detected over the putative regulatory regions of pgk1⁺, fba1⁺, bip1⁺, act1⁺, adh1⁺, SPBC8E4.01c⁺, and ssa2⁺. The coordinates of the regions that were tested are provided in Supplementary Table 6. The background %IP values obtained from ChIP analysis of the corresponding ‘no tag’ strains are shown in Supplementary Figure S8A and B. Each column represents the mean relative %IP value±s.e. (n=4).