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. 2011 Jun 10;30(14):2894–2907. doi: 10.1038/emboj.2011.184

Figure 2.

Figure 2

E4f1 modulates Wnt-dependent regulation of cdx4 and hoxa9a expression. (A–H) cdx4 expression in the caudal region at the end of gastrulation is reduced by inhibiting Wnt signalling and further attenuated by e4f1 depletion. (I–R) Expression of hoxa9a at the end of gastrulation is likewise regulated by Wnt signalling and E4f1. Injected reagents are listed at the bottom, probe for in situ hybridization at top right. wnt MO mix indicates a mixture of wnt3a MO (2 ng), wnt8.1 MO (2 ng) and wnt 8.2 MO (2 ng); 5 ng of e4f1 MO and 25 pg of dkk1 mRNA were injected. (A–M) Lateral view, anterior is up and dorsal to the right. (N–R) Posterior view, dorsal is up. (S–U) E4f1 acts downstream of Cdx4. E4f1 mRNA (150 pg) does not rescue tail defects caused by cdx4 MO (2 ng). Scale bars, 200 μm.