Figure 3.

Interaction between FliM and FliG_C detected in GST pull-down assays. (Representative results are shown; see Supplementary Figure S2 for additional data.) Blots were probed with anti-FliM antibody. (A) Effects of FliG_C mutations on the binding to FliM. Positions where mutations eliminated binding are coloured red; black indicates positions where mutations had no effect. (B) Effects of FliM mutations on binding to FliG_C. Colouring as in panel (A), plus orange to indicate positions where binding was weakened.