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. 2011 Jun 14;30(14):2779–2792. doi: 10.1038/emboj.2011.197

Figure 2.

Figure 2

BCL-XL interacts with Ybh3p and inhibits Ybh3p-mediated death. (A, B) Survival determined by clonogenicity (A) and quantification of ROS accumulation using DHE → Eth conversion (B) of cells overexpressing Ybh3p alone or co-expressing Ybh3p and BCL-XL and the corresponding vector controls upon treatment with or without 120 mM acetic acid. Survival was normalized to untreated control cultures (mean±s.e.m., n=5). (C) Protein extracts of cells expressing BCL-XL either alone or in combination with FLAG-tagged Ybh3p as well as of cells harbouring both empty vectors were used to immunoprecipitate FLAG-tagged Ybh3p using agarose beads coupled with a monoclonal anti-FLAG antibody. Immunoprecipitates (eluates) as well as whole protein extracts were analysed via immunoblot for BCL-XL and Ybh3p. (D, E) Survival determined by clonogenicity (D) and quantification of ROS accumulation using DHE → Eth conversion (E) of WT and Δuth1 cells overexpressing Ybh3p or harbouring the empty vector upon treatment with or without 120 mM acetic acid or 0.6 mM H2O2. Survival was normalized to untreated isogenic vector control (mean±s.e.m., n=6). (F) Immunoblot analysis of WT and Δuth1 cells for FLAG-tagged Ybh3p. **P<0.01 and ***P<0.001.