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. 2011 Aug 23;6(8):e22993. doi: 10.1371/journal.pone.0022993

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Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The expanded region (m/z 3650–5000) is shown on the panels D, (AB2.2), E, (NN5) and F, (F10). N-glycans were permethylated prior being subjected to MS analysis and m/z values correspond to [M+Na]⁺ ions. Scheme assignments are based on the precise fit between composition calculations and the m/z (z = 1) ratio of the molecular ions detected. These schemes represent the most likely structures taking into account the biosynthetic pathways and the enzyme repertoire of murine cells and selected MS/MS analyses. Sugar symbols are those employed by the Consortium for Functional Glycomics. Circles represent hexoses (yellow: galactose, green: mannose), blue squares represent N-acetylglucosamine, red triangle: fucose, purple diamonds represent N-acetylneuraminic acid.