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. 2011 Aug 23;6(8):e22653. doi: 10.1371/journal.pone.0022653

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Samples of whole blood from HIV-1 infected and uninfected individuals were analysed using flow cytometry and the MultiTEST™ IMK Kit (BD). Lymphocyte subpopulations were quantified and samples from 261 HIV-1 negative individuals (white box) were compared with HIV positives. The absolute counts of CD19+ B lymphocytes in 50 HIV-1 subtype A infected patients (grey box) and in 192 patients with all HIV-1 subtypes combined (hatched box) were significantly reduced in comparison to uninfected participants. (p<0.0001, Mann-Whitney test). The whiskers represent the range from the 10th–90th percentile.