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. 2011 Aug 23;6(8):e23732. doi: 10.1371/journal.pone.0023732

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Chico et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The indicated strains (all Uri⁺ and all derived from CAI4) were passaged on YPD plates by successively streaking for single colonies. Chromosomal DNA samples were prepared from the strains following 2, 4, 6 and 8 streaks, and subjected to telomere Southern analysis as described [34].