Effect of TGFβ and FGF on PE explants. A, Representative TGFβ2- and vehicle-incubated PE explants photographed at 24, 48 and 72 hours after harvest. Explants incubated with 200 pM TGFβ2 contain more elongate cells radiating from the explant (arrowhead) as compared to vehicle incubated explants. B, The number of transformed cells was increased significantly in explants incubated with 200 pM of either TGFβ1 or TGFβ2, *=p<0.05. C, EMT in PE explants incubated with 10 ng/ml FGF1 or FGF7 did not differ from vehicle incubated explants. All photomicrographs at 100x.