Figure 4.

Effect of glycerol concentration on retinoid production. Retinoid (retinal, retinol, and retinyl acetate) production and cell growth of E. coli harboring pT-DHBSR and pS-NA. Culture was carried out in 2YT medium containing 0%, 0.5%, 1%, and 2% (w/v) glycerol and 0.2% (w/v) arabinose at 29°C. For retinoid production, retinal, retinol, and retinyl acetate are indicated with light gray, dark gray, and black, respectively. For cell growth, the glycerol concentrations are indicated as symbols; 0%, squares; 0.5%, circles; 1%, triangles; 2%, reversed triangles.