Figure 5.

Effect of dodecane overlay on retinoid production. Retinoid production and cell growth of E. coli (pT-DHBSR/pS-NA) in two-phase culture system using a 1 mL of dodecane overlay and 5 mL of culture broth. The culture was carried out in 2YT medium containing 0.5%, 1%, and 2% (w/v) glycerol and 0.2% (w/v) arabinose at 29°C. For retinoid production, retinal, retinol, and retinyl acetate are indicated with light gray, dark gray, and black, respectively. For cell growth, the glycerol concentrations are indicated as symbols; 0.5%, squares; 1%, circles; 2%, triangles.