Figure 5.

Effect of NVP-BEZ235 (NVP) alone or in combination with Sorafenib (Soraf) on tumor cell proliferation, apoptosis and tumor angiogenesis. A, Frozen sections of tumor xenografts treated as in Figure 4 and harvested after 20 days of treatment were stained with an anti-Ki-67 antibody. The effect of the different treatments on Ki-67 positivity was quantified and expressed as % of cells positive for Ki-67/total number of cells (300 cells counted per tumor; five tumors in each group). Columns, mean % of cells positive for Ki-67 staining; bars, SD. B, Tumor lysates were generated from xenografts and analyzed for cleaved caspase-3 and actin expression. C, Frozen sections of tumor xenografts were stained with an anti-CD31 antibody. Vessels were manually counted in five high-power fields (HPF) in each tumor. Columns, mean vessel number per HPF (three tumor xenografts analyzed in each group); bars, SD. *, P < 0.05, #, P < 0.001 compared to control.