Skip to main content
. Author manuscript; available in PMC: 2011 Aug 23.
Published in final edited form as: J Appl Microbiol. 2007 Oct;103(4):871–881. doi: 10.1111/j.1365-2672.2007.03319.x

TABLE 2.

Average detection rates by CARD-FISH for DKAS 1, LKAS 2, HKAS 3 and SKAS 9 (n = 56; 7 sampling dates, four springs, two replicates per sample).

Date Total cell
counts
[104cells ml−1]
Fraction % of total cells detected with probe*
% of total
hybridization§
Range
min - max
EUB338 EUB-mix ΔEUB EURY806 CREN537 NON338
07.01.04 3.4 59 74 15 6 4 1 82 74 – 96
02.02.04 2.4 n.a** 73 n.a. 7 1 0.6 80 55 - 94
25.02.04 2.1 n.a. 62 n.a. 10 2 0.3 74 64 - 84
23.03.04 2.6 63 74 11 4 5 0.5 82 67 - 96
19.04.04 3.1 n.a. 79 n.a. 3 3 0.7 85 83 - 88
17.05.04 3.0 75 80 6 4 3 0.4 86 81 - 94
14.06.04 2.5 n.a. 82 n.a. 5 4 0.5 91 82 - 99

Avg. †† 2.7 66 75 10 6 3 0.6 83 55 - 99
*

Percent detection of DAPI-stained cells. Between 800 and 900 DAPI-stained cells were counted per filter piece. Average values based on 8 analysis.

Hybridization with a mixture of probes EUB338, EUB338 II and EUB338 III.

Difference between fraction (%) EUB338 and EUBmix.

§

Percentage of total hybridization of probes (EUBmix, EURY806, CREN537) normalized to total DAPI counts. Numbers have been corrected by substracting NON338 counts.

**

not analysed

††

total average