Table 1.
Reverse transcription-polymerase chain reaction primer sequences
| Primer | Sequence (5’-3’) | Product size (bp) | Temperature (°C) |
| Notch1-ICD | Forward: CTAAGATCTCCTGAGGGCTTCAAAGTGTC | 2375 | 63 |
| Reverse: GCGAATTCCTTGAAGGCCTCCGGAAT | |||
| H-Ras (ORF) | Forward: GAGGATCCATGACGGAATATAAGCTGG | 668 | 61 |
| Reverse: GTGAATTCTCAGGAGAGCACACACTTG | |||
| Hes-1 | Forward: ATGAACGAGGTGACCCGCTT | 443 | 62 |
| Reverse: CTGGAAGGTGACACTGCGTT | |||
| LSF (ORF) | Forward: GGAAAGATCTAGGATGGCCTGGGCTCTGAAG | 1547 | 59 |
| Reverse: CAAAGCTTGGGCACGAAACGCCGCACTCCT | |||
| LSF (promoter analysis) | Forward: CTAGGTACCCAACATGGTAAGATCCTGTCTCT | 2020 | 56 |
| Reverse: GGAGATCTTCTCATCCCTGCTTTCTGTTTCCT | |||
| GAPDH | Forward: GGTGGAGGTCGGAGTCAACGGA | 240 | 60 |
| Reverse: GAGGGATCTCGCTCCTGGAGGA |
ORF: Open-reading frame; ICD: Intracellular domain; LSF: Late SV40 factor; LSF (promoter analysis): Upstream sequence before start code.