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. 2011 Aug 22;194(4):643–656. doi: 10.1083/jcb.201104124

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© 2011 Taguchi et al.

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Figure 1. — Two types of AJ in a DLD1 colony. (A) Projected confocal images of a colony of DLD1 cells double-immunostained for E-cadherin and F-actin. Arrowheads and arrows point to linear and punctate portions of the AJ outlining a peripheral cell, respectively. Asterisk, a putative ZA remnant. (B) The same image as in A, but focused on apical and basal planes of the colony, highlighting ZAs in inner cells and pAJs in peripheral cells, respectively. At the ZAs, linear E-cadherin and F-actin signals closely colocalize with each other, whereas E-cadherin signals at pAJs are associated with the terminals of peripheral actin fibers. (C) Projected confocal images of myosin IIA or IIB, immunostained together for E-cadherin. Both isoforms delineate ZAs. In addition, myosin IIA decorates peripheral F-actins, and IIB organizes a closed ring even in peripheral cells. (D) The small arrow points to a pAJ of a colony, and the large arrow indicates a contact point between this colony and another colony. Note that these two junctions were morphologically similar to one another. Bars, 10 µm.