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. 2011 Aug 22;194(4):551–565. doi: 10.1083/jcb.201010090

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© 2011 Tao et al.

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Figure 6. — Set7 is required for MyoD to bind to the promoters/enhancers of skeletal muscle differentiation genes. (A) C2C12 myoblasts were transfected with control siRNA or siRNA against Set7. Cells were transferred to differentiation medium for the indicated times and Western blot analyses were performed with cell extracts using antibodies that recognized Set7 proteins. β-Tubulin was used as a loading control. (B) Schematic diagrams of promoter or enhancer regions of indicated myogenic differentiation genes. Arrows indicate PCR primers used for the PCR reaction. (C) IPs were performed with anti-Set7 and anti–H3-K4 antibodies as indicated. (D) IPs were performed with anti-MyoD antibodies. (C and D) The amount of DNA in each sample (input) is shown. IPs performed with IgG were used as controls. GAPDH was used as a loading control. Myog, myogenin; Crtl. control; D0, day 0.