Figure 2.

H&E and IHC staining for inter-leukin (IL)-17 in histological patterns of formalin-fixed paraffin-embedded whole mount radical prostatectomy from patients with prostate cancer. A. scattered stromal IL-17 expressing cells (brown color, red arrowheads, ×125 and red arrows, ×800) in benign prostate tissue distant from cancer; B. scattered stromal IL-17 expressing cells (red arrowheads, ×125) and scattered IL-17 expressing cells in mononuclear cell (MNC) hot spot (red arrows, ×800) within a focus of ade-nocarcinoma; C. scattered stromal IL-17 expressing cells (red arrowheads, ×125) and IL-17 expressing cells (red arrow, ×800) in a MNC hot spot in benign tissue adjacent to a focus of adenocarcinoma. IL-17 expressing cells with monocyte/ macrophage morphology are present within a capillary lumen (red circle) at the edge of the hot spot; D. scattered stromal IL-17 expressing cells (red arrowheads, ×125) and IL-17 expressing cell (red arrow, ×800) in a MNC hot spot in benign tissue distant from cancer; E. stromal (red arrowheads, ×125), peri-glandular (black arrowhead, ×800) and intra-glandular (black dotted circles, ×125 and red arrows, ×800) accumulation of IL-17 expressing cells (brown color) in a proliferative inflammatory atrophy (PIA) lesion. Left column shows H&E and immunohistochemical staining for IL-17 in various histological patterns at magnification ×125. Right column depicts H&E and immunohistochemical staining for IL-17 in tissues located inside the black squares of the left columns of the same samples with magnification ×800. The images illustrate scant numbers of IL-17 expressing cells scattered in benign prostate tissue (A) and in MNC hot spots (B, C, D) in various his-tologic zones. The most striking accumulations of IL-17 expressing cells appear within the lumens of glands in areas of PIA lesions in the peripheral zone of the prostate (E). Details are described in “Materials and Methods” section. (Figure 2D and E on next page).