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. 2011 Aug 3;4(6):552–565.

Figure 4.

Figure 4

H&E and IHC staining for IL-17, CD3, CD20, CD4, and CD68, in the PIA lesion of formalin-fixed paraffin-embedded whole mount radical prostatectomy specimens from patients with prostate cancer. Consecutive prostate tissue slide staining for: A - H&E, B - IL-17 (brown color), C - two color CD3 (T lymphocytes - pink color) and CD20 (B lymphocytes - brown color), D - CD3 (pink color) and CD4 (brown color) with co-localization of CD3 and CD4 (dark brown or black colors) on helper-inducer T lymphocytes (dark brown or black colors), and E - IL-17 (brown color) and CD68 (pink color) with co-expression of IL-17 and CD68 proteins in tissue macrophages (dark brown or black colors). Red arrows (x800) indicate intra-glandular IL-17 expressing cells. Yellow asterisks (x125) indicate sites of B and T cell accumulation in MNC hot spots in the prostate stroma. Black arrowheads (x800) indicate peri-glandular and glandular T cells. Green arrowheads (x800) indicate peri-glandular CD4+CD3+ T helper cells. Black arrows (x800) indicate peri-glandular CD3 T cells without co-localization of CD4 antigen. Red arrowheads (x800) indicate intra- glandular IL-17 producing CD68 positive macrophages. Left column presents H&E staining and leukocyte immunophenotypingof the same PIA lesion with magnification ×125. Right column depicts H&E staining and leukocyte immunophenotyping of tissue of the same PIA lesion located inside of the black squares of the left columns with magnification ×800. The figure shows that IL-17 positive cells (B) are located predominantly intraluminally in PIA lesions and that they are not T or B lymphocytes (C), or classical IL-17 expressing CD4+CD3+ T helper (TH17) cells (D), but rather CD68+ tissue macrophages (E) expressing IL-17 protein. Details are described in “Materials and Methods” section.