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Sensitivity and specificity of miRNA quantification by a SYBR Green-based real-time RT-PCR (qRT-PCR) method. (A) Standard curves of miR-155, miR-197, and miR-182. The assay showed high sensitivity and a broad dynamic range. (B) Discrimination power (specificity) of the assay on let-7 miRNA family members. Relative detection (%) was calculated based on Ct value difference between perfectly matched and mismatched targets let-7a and let-7b.
