Fig. 1.

Schematic of capture strategy. Overlapping oligonucleotides flanked by universal sequences complimentary to the 169 genes listed in table S1 were synthesized on an array. The oligonucleotides were cleaved off the array, amplified by PCR with universal primers, ligated into concatamers, and amplified in an isothermal reaction. They were then bound to nitrocellulose filters and used as bait for capturing the desired fragments. An Illumina library was constructed from the sample DNA. The library was denatured and hybridized to the probes immobilized on nitrocellulose. The captured fragments were eluted, PCR-amplified, and sequenced on an Illumina GAIIX instrument.