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. 2011 Jul 8;30(16):3298–3308. doi: 10.1038/emboj.2011.228

Figure 5.

Figure 5

Tdrd1-associated piRNAs. (A) Radiolabelled RNA from the indicated IPs was separated on a denaturing gel, followed by autoradiography. Bracket indicates piRNAs. The solid bar indicates Tdrd1-associated transcripts (TATs). Arrows indicate rRNA species. (B) Length distributions of piRNAs from Ziwi, Zili and Tdrd1 IPs. (C) Heatmap showing strand biases of piRNAs from individual transposons in the indicated libraries. Map is relative from left to right and is sorted on strand ratios of Tdrd1-associated piRNAs. Low Z-score (red) indicates relative enrichment for antisense reads. (D) Scatterplot displaying the coverage of different transposon types (open circles) by piRNAs from a Zili IP (X axis) and a Tdrd1 IP (Y axis). Correlation coefficient R=0.93.