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TorsinA's NTD can be converted into a TMD. (A) MPEx predicted TM segment in torsinA and ΔGpred for membrane insertion of wild-type and mutated NTD sequences. (B–F) Epifluorescence microscopy of cells expressing the indicated GFP fusion proteins, stained with anti-GFP antibody after either selective permeabilization in digitonin (B–F) or complete permeabilization in Triton-X-100 (B′–F′). Scale bars, 10 μm.
