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. 2011 Jul 19;30(16):3430–3441. doi: 10.1038/emboj.2011.240

Figure 1.

Figure 1

cyca2 triple mutants have defects in cell-cycle progression. (A) Propidium iodide stained root meristems of WT, cyca2;124 (cyca2;1-1 cyca2;2-1 cyca2;4-1), cyca2;134 (cyca2;1-1 cyca2;3-1 cyca2;4-1) and cyca2;234 (cyca2;2-1 cyca2;3-1 cyca2;4-1) 10 days after germination. Arrowheads indicate the ends of meristems, defined as the position where cells start elongating. (B) Immunolocalization of the cytokinesis-specific syntaxin KNOLLE, labelling cells undergoing cytokinesis in roots of 7-day-old WT, cyca2;124, cyca2;134 and cyca2;234. (C) Stage II and stage V lateral root primordia of WT and cyca2;234 cleared with chloral hydrate. Lateral root primordia of cyca2;234 are composed of fewer cells than WT. Arrowheads indicate periclinal cell walls. Stages as defined previously (Malamy and Benfey, 1997). (D) Transcriptional responses of auxin signalling genes, G1/S and G2/M regulators in WT and cyca2;234 root segments during auxin-induced lateral root initiation. In all, 0, 2 and 6 h correspond to time of auxin treatment (10 μM) after being germinated in presence of the auxin transport inhibitor NPA (10 μM). Range indicator from blue to yellow represents expression levels on a log2 scale relative to NPA germinated WT (0 h).